Name : Human AGER Protein

Product Source :
Recombinant Human AGER Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala23- Ala344.[Accession | Q15109-1]

Molecular Weight :
The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human AGER on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human AGER is greater than 95% as determined by SEC-HPLC.

Background :
The receptor for advanced glycation end products (AGER) is an oncogenic transmembranous receptor up-regulated in various human cancers. AGER promotes proliferation, migration, and inhibits apoptosis of squamous cervical cancer and might function as a tumor promoter in cervical cancer. Our study provides novel evidence for a potential role of AGER in bridging human papillomavirus (HPV)-induced inflammation and cervical cancer.

Synonyms :
AGER; RAGE; SCARJ1

References & Citations :
(1) Zhu X, Zhou L, Li R, Shen Q, Cheng H, Shen Z, Zhu H. AGER promotes proliferation and migration in cervical cancer. Biosci Rep. 2018 Jan 30;38(1):BSR20171329. doi: 10.1042/BSR20171329. PMID: 29298878; PMCID: PMC5789157.

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