Name : Cynomolgus TPBG/5T4 Protein

Product Source :
Recombinant Cynomolgus TPBG/5T4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr35-Ser355.[Accession | Q4R8Y9]

Molecular Weight :
The protein has a predicted MW of 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus TPBG is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus TPBG, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.

Background :
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.

Synonyms :
M6P1; 5T4AG; WAIF1; TPBG; 5T4

References & Citations :
(1) Harrop R, O’Neill E, Stern PL. Cancer stem cell mobilization and therapeutic targeting of the 5T4 oncofetal antigen. Ther Adv Vaccines Immunother. 2019 Jan 25;7:2515135518821623. doi: 10.1177/2515135518821623. PMID: 30719508; PMCID: PMC6348545.

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