Name : Biotinylated Human CD155/PVR Protein

Product Source :
Recombinant Biotinylated Human CD155/PVR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Trp21-Asn343.[Accession | P15151-1]

Molecular Weight :
The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human CD155 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.

Background :
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the “poliovirus receptor,” or PVR.

Synonyms :
CD155; HVED; NECL5; Necl-5; nectin-like 5; PVR; PVS; Tage4; PVSFLJ25946; FLJ25946

References & Citations :
(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

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