Name : Human CD21 Protein
Product Source :
Recombinant Human CD21 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile21-Arg971.[Accession | P20023-1]
Molecular Weight :
The protein has a predicted MW of 105.3 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD21 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD21, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD21 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
Background :
CD21 mRNA expression, an EBV-entry receptor, was transiently detected in NK cells after exosome treatment, and levels decreased after further culture. CD21 protein expression was also transiently transferred to NK cells after co-culture with an EBV-positive Burkitt lymphoma cell line (Raji) via trogocytosis. However, EBV did not infect NK cells through CD21-mediated trogocytosis.
Synonyms :
EBV receptor; CD21; C3DR; CR2; C3DRSLEB9; CVID7; SLEB9
References & Citations :
(1) Lee JH, Choi J, Ahn YO, Kim TM, Heo DS. CD21-independent Epstein-Barr virus entry into NK cells. Cell Immunol. 2018 May;327:21-25. doi: 10.1016/j.cellimm.2018.01.011. Epub 2018 Jan 17. PMID: 29499908.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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