Name : Biotinylated Human CD24 Protein
Product Source :
Recombinant Biotinylated Human CD24 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser27-Gly59.[Accession | P25063-1]
Molecular Weight :
The protein has a predicted MW of 6.5 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human CD24 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD24 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human CD24, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.
Background :
CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.
Synonyms :
CD 24; CD24 molecule; CD24A; FLJ22950; FLJ43543; MGC75043
References & Citations :
(1)Barkal AA, et al.CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.Nature. 2019 Jul 31. doi: 10.1038/s41586-019-1456-0.D
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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