Name : Biotinylated Human CD38 Protein

Product Source :
Recombinant Biotinylated Human CD38 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Val43-Ile300.[Accession | P28907-1]

Molecular Weight :
The protein has a predicted MW of 32.50 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human CD38 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD38 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-CD38 Antibody, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CD38, His Tag with the EC50 of 0.9μg/ml determined by ELISA.

Background :
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling.

Synonyms :
ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule; EC 2.4.99.20; EC 3.2.2.6

References & Citations :
(1)Alba MatasCéspedes, Vidalcrespo A , Rodriguez V , et al. Daratumumab, a Novel Human Anti-CD38 Monoclonal Antibody for the Treatment of Chronic Lymphocytic Leukemia and B-Cell Non–Hodgkin Lymphoma[J]. Blood, 2012.

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