Name : Biotinylated Human Siglec-3/CD33 Protein
Product Source :
Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp18-His259.[Accession | P20138-1]
Molecular Weight :
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Supplied as 0.22μm filtered solution in 50m MES, 500mM NaCl, 200mM L-arginine (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Siglec-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human Siglec-3 is greater than 90% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Background :
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Synonyms :
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
References & Citations :
(1)Walter R B , Appelbaum F R , Estey E H , et al. Acute myeloid leukemia stem cells and CD33-targeted immunotherapy[J]. Blood, 2012, 119(26):6198-6208.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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