Name : Mouse CD31/PECAM-1 Protein
Product Source :
Recombinant Mouse CD31/PECAM-1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu18-Lys590.[Accession | Q08481-1]
Molecular Weight :
The protein has a predicted MW of 65.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CD31/PECAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CD31/PECAM-1 is greater than 95% as determined by SEC-HPLC.
Background :
Platelet endothelial cell adhesion molecule 1 (PECAM-1) is an adhesion and signaling receptor that is expressed on endothelial and hematopoietic cells and plays important roles in angiogenesis, vascular permeability, and regulation of cellular responsiveness.
Synonyms :
PECAM-1; EndoCAM; PECA1; CD31; FLJ34100; FLJ58394; GPIIA’
References & Citations :
(1) Zhi H, Kanaji T, Fu G, Newman DK, Newman PJ. Generation of PECAM-1 (CD31) conditional knockout mice. Genesis. 2020 Feb;58(2):e23346. doi: 10.1002/dvg.23346. Epub 2019 Nov 15. PMID: 31729819; PMCID: PMC7021573.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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