Name : Human CD46 Protein
Product Source :
Recombinant Human CD46 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys35-Asp343.[Accession | P15529-1]
Molecular Weight :
The protein has a predicted MW of 35.4 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD46 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD46 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD46, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
Background :
CD46 was discovered in 1986 during a search for novel C3b-binding proteins. CD46 is expressed ubiquitously and functions as a co-factor in the factor I-mediated proteolytic cleavage of C3b and C4b. Its vital role in preventing complement deposition on host tissue is underpinned by the fact that deficiency of CD46 is a predisposing factor for numerous disease conditions arising from complement-mediated ‘self-attack’.
Synonyms :
TLX; CD46; MCP; MIC10; AHUS2; TRA2.10
References & Citations :
(1) Cardone J, Le Friec G, Kemper C. CD46 in innate and adaptive immunity: an update. Clin Exp Immunol. 2011 Jun;164(3):301-11. doi: 10.1111/j.1365-2249.2011.04400.x. Epub 2011 Apr 13. PMID: 21488871; PMCID: PMC3087925.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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