Name : Biotinylated Mouse CD5 Protein (Primary Amine Labeling)

Product Source :
Recombinant Biotinylated Mouse CD5 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus. It contains Ser25-Pro371.[Accession | P13379]

Molecular Weight :
The protein has a predicted MW of 38.9 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Mouse CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Mouse CD5 is greater than 95% as determined by SEC-HPLC.

Background :
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.

Synonyms :
CD5 molecule; CD5; LEU1; T1; CD5 antigen

References & Citations :
(1)Jevremovic, D., Dronca, R. S., Morice, W. G., McPhail, E. D., Kurtin, P. J., Zent, C. S., & Hanson, C. A. (2010). CD5 B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Leukemia Research, 34(9), 1235-1238.

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