Name : Biotinylated Human CD7 Protein
Product Source :
Recombinant Biotinylated Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala26-Pro180.[Accession | P09564]
Molecular Weight :
The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human CD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD7 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human CD7, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
Background :
CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily.CD7 is expressed on T cells, NK cells , myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells.
Synonyms :
CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40
References & Citations :
(1)Gomes-Silva D , Srinivasan M , Sharma S , et al. CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies[J]. Blood, 2017:blood-2017-01-761320.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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