Name : Human CD83 Protein
Product Source :
Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr20-Ala143.[Accession | Q01151-1]
Molecular Weight :
The protein has a predicted MW of 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD83 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD83 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD83, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.
Background :
CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.
References & Citations :
(1) Li Z, Ju X, Silveira PA, Abadir E, Hsu WH, Hart DNJ, Clark GJ. CD83: Activation Marker for Antigen Presenting Cells and Its Therapeutic Potential. Front Immunol. 2019 Jun 7;10:1312. doi: 10.3389/fimmu.2019.01312. PMID: 31231400; PMCID: PMC6568190.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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