Name : Human CD8 alpha&beta Heterodimer Protein
Product Source :
Recombinant Human CD8 alpha&beta Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus. It contains Ser22-Asp182(CD8 alpha) acidic tail and Leu22-Pro170(CD8 beta) basic tail.[Accession | P01732-1(CD8 alpha)&P10966-1(CD8 beta)]
Molecular Weight :
The protein has a predicted MW of 23.54 kDa (CD8 alpha) and 22.62 kDa (CD8 beta). Due to glycosylation, the protein migrates to 26-28 kDa (CD8 alpha) and 28-32 kDa (CD8 beta) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD8 alpha&beta Heterodimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD8 alpha&beta Heterodimer is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD8A&CD8B, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD8 Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.
Background :
CD8 alpha&beta (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR.
Synonyms :
CD8 alphaβ CD8A&CD8B;
References & Citations :
[1] Nomura A, Taniuchi I. The Role of CD8 Downregulation during Thymocyte Differentiation. Trends Immunol. 2020 Nov;41(11):972-981.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
DSG3 ProteinSource
PIGR ProteinPurity & Documentation
Popular categories:
Cystatin Family
IFN-alpha 2a