Name : Biotinylated Human CD93/C1q R1 Protein
Product Source :
Recombinant Biotinylated Human CD93/C1q R1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr22-Lys580.[Accession | Q9NPY3]
Molecular Weight :
The protein has a predicted MW of 61.2 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human CD93 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD93 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human CD93, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IGFBP7, hFc Tag with the EC50 of 27.9ng/ml determined by ELISA (QC Test). SPR Data Biotinylated Human CD93, His-Avi Tag immobilized on CM5 Chip can bind Human IGFBP-7, His Tag with an affinity constant of 0.17 μM as determined in SPR assay (Biacore T200).
Background :
CD93 has been shown critical roles in inflammatory and immune diseases. CD93 silencing increased IL-6 and TSLP, but not IL-33 levels in culture supernatants. HDM-induced asthma mice showed significant airway hyperresponsiveness and inflammation with Th2 cytokine activation, along with decreased CD93 expression in bronchial epithelial cells and lung homogenates but increased serum CD93 levels.
Synonyms :
C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qRp; CDw93; CD93; C1qR1; MXRA4; ECSM3; dJ737E23.1
References & Citations :
(1) Park HJ, Oh EY, Han HJ, Park KH, Jeong KY, Park JW, Lee JH. Soluble CD93 in allergic asthma. Sci Rep. 2020 Jan 15;10(1):323. doi: 10.1038/s41598-019-57176-2. PMID: 31941986; PMCID: PMC6962376.
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