Name : Human CD1a Protein
Product Source :
Recombinant Human CD1a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp19-Val300.[Accession | NP_001754.2 ]
Molecular Weight :
The protein has a predicted MW of 33.3 kDa. Due to glycosylation, the protein migrates to 48-53 kDa and 13-14 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD1a on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD1a is greater than 95% as determined by SEC-HPLC.
Background :
CD1 proteins are a family of major histocompatibility complex (MHC) class I-like antigen-presenting molecules that present lipids to T cells. The cytoplasmic tails (CTs) of all human CD1 isoforms, with the exception of CD1a, contain tyrosine-based sorting motifs, responsible for the internalization of proteins by the clathrin-mediated pathway. CD1a closer to MHC class I in its trafficking and potential antigen-loading compartments among CD1 isoforms.
References & Citations :
(1) Barral DC, Cavallari M, McCormick PJ, Garg S, Magee AI, Bonifacino JS, De Libero G, Brenner MB. CD1a and MHC class I follow a similar endocytic recycling pathway. Traffic. 2008 Sep;9(9):1446-57. doi: 10.1111/j.1600-0854.2008.00781.x. Epub 2008 Jun 28. PMID: 18564371; PMCID: PMC3839101.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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