Name : Human CLEC9A Protein
Product Source :
Recombinant Human CLEC9A Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Lys57-Val241.[Accession | Q6UXN8]
Molecular Weight :
The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CLEC9A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CLEC9A is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CLEC9A, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Background :
CLEC9A expression was significantly higher in psoriatic skin compared with healthy donor. In psoriatic skin and PsA ST, CLEC9A() cells were in close proximity to TUNEL() cells. SF CLEC9A levels were significantly lower compared with paired PsA serum. Adalimumab treatment did not affect CLEC9A serum level and skin expression. The downregulation of synovial CLEC9A might be associated with a novel mechanism by which anti-TNF therapy might reduce CD8-mediated inflammation in PsA patients.
Synonyms :
CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341
References & Citations :
(1) Ramos MI, Teunissen MB, Helder B, Aarrass S, de Hair MJ, van Kuijk AW, Gerlag DM, Tak PP, Lebre MC. Reduced CLEC9A expression in synovial tissue of psoriatic arthritis patients after adalimumab therapy. Rheumatology (Oxford). 2016 Sep;55(9):1575-84. doi: 10.1093/rheumatology/kew204. Epub 2016 May 13. PMID: 27179104.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD164 ProteinSynonyms
PDCD4 Proteinmedchemexpress
Popular categories:
Inhibin B
Cholinergic Receptor Muscarinic 1 (CHRM1)