Name : Cynomolgus CTLA-4/CD152 Protein
Product Source :
Recombinant Cynomolgus CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala37-Ser160.[Accession | G7PL88-1]
Molecular Weight :
The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus CTLA-4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA (QC Test). SPR Data Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).
Background :
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an “off” switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
Synonyms :
CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
References & Citations :
(1)Merwe V D , P. A. CD80 (B7-1) Binds Both CD28 and CTLA-4 with a Low Affinity and Very Fast Kinetics[J]. Journal of Experimental Medicine, 1997, 185(3):393-404.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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