Name : Human DLL1/Delta1 Protein
Product Source :
Recombinant Human DLL1/Delta1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln18-Gly540.[Accession | NP_005609]
Molecular Weight :
The protein has a predicted MW of 57.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human DLL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human DLL1 is greater than 95% as determined by SEC-HPLC.
Background :
DLL1, a Notch signaling ligand, is significantly overexpressed in ERα luminal breast cancer. Intriguingly, DLL1 overexpression correlates with poor prognosis in ERα luminal breast cancer, but not in other subtypes of breast cancer. In addition, this effect is specific to DLL1, as other Notch ligands (DLL3, JAGGED1, and JAGGED2) do not influence the clinical outcome of ERα patients. DLL1-mediated Notch signaling in breast cancer is important for tumor cell proliferation, angiogenesis, and cancer stem cell function.
Synonyms :
H-Delta-1; DLL1; DELTA1; DL1
References & Citations :
(1) Kumar S, Srivastav RK, Wilkes DW, Ross T, Kim S, Kowalski J, Chatla S, Zhang Q, Nayak A, Guha M, Fuchs SY, Thomas C, Chakrabarti R. Estrogen-dependent DLL1-mediated Notch signaling promotes luminal breast cancer. Oncogene. 2019 Mar;38(12):2092-2107. doi: 10.1038/s41388-018-0562-z. Epub 2018 Nov 15. PMID: 30442981; PMCID: PMC6756232.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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