Name : Human DLL4 Protein
Product Source :
Recombinant Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ser27-Pro524.[Accession | Q9NR61]
Molecular Weight :
The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human DLL4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human DLL4, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Human Notch 1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. SPR Data Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 μM as determined in SPR assay (Biacore T200).
Background :
Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
Synonyms :
DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
References & Citations :
(1)Gurney A , Hoey T. Anti-DLL4, a cancer therapeutic with multiple mechanisms of action[J]. Vascular Cell, 2011, 3(1):18.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
Arginase-1/ARG1 ProteinStorage & Stability
B7-2/CD86 Proteinmanufacturer
Popular categories:
Mannose-binding Protein C
TRIM/RBCC Proteins