Name : Human EGF Protein
Product Source :
Recombinant Human EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Asn971-Arg1023.[Accession | P01133-1]
Molecular Weight :
The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. The purity of Human EGF is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human EGFR, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA. SPR Data Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). Cell Based Assay Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 – 1.5 ng/mL (QC Test).
Background :
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Synonyms :
HOMG4; EGF; URG
References & Citations :
(1)Normanno N, Bianco C, De Luca A, Salomon DS. The role of EGF-related peptides in tumor growth. Front Biosci. 2001 May 1;6:D685-707. doi: 10.2741/normano. PMID: 11333208.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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