E). The largest group of strains hypersensitive to CTBT contained deletions in genes for mitochondrial biogenesis and functions, like DNA replication (MIP), mRNA processing (MRS), protein synthesis and processing (AFG, DIA, MRPL, MRPS, MSY, MTG, OCT, PCP, RML, RSM, TUF), respiration (CYT), ATP synthesis (ATP, ATP, ATP, ATP), FeS protein biosynthesis (ISA, ISA), superoxide buy Docosahexaenoyl ethanolamide dismutation (SOD, SOD) and others. Inside the second largest group have been mutants in genes inved in gene expression therefore hinting at an acute transcriptional response to CTBT. Identified genes are inved in chromatin remodeling (ARP, HOS, HTZ, RSC, SGF, SNF, SWI, SWI, YAF), transcription (CTK, MED, ROX, RRN, RTF, SPT, SPT, SRB, TAF, THO) or encode transcription factors inved in oxidative tension response (YAP, YAP, SKN) and lipid biosynthesis (OPI). We identified at the very least CTBT sensitive strains containing deletion in DNA repair genes, such as these inved in homologous recombination and repair (MMS, MMS, RAD, RAD, RAD, RAD), post replication repair (RAD, RAD), double strand break repair (MRE, XRS), excision repair (MET) and other folks. Importantly, deletion of genes encoding functions in lipid metabolism also impaired CTBT tolerance. These are inved in ergosterol (DAP, ERG, ERG,Table Functions of selected genes deleted 
in CTBT sensitive mutant strainsFunction Mitochondrial functions Chromatin remodeling and transcription DNA repair Lipid metabolism Tension response and PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27382054?dopt=Abstract signal transduction Vacuolar functions Protein sorting and degradation Amino acid metabolism Transport Mannosyl transferases Pentose phosphate pathway Ungrouped Gene (ORF) name ADH, AFG, ATP, ATP, ATP, ATP, CIT, CYT, DIA, IMP, ISA, ISA, MAS, MDM, MDM, MGM, MIP, MRPL, MRPS, MRS, MSY, MTG, OCT, PCP, PDC, PHB, RML, RSM, SOD, SOD, TUF, YDRW, YGLW ARP, CDC, CTK, HOS, HPR, IES, MED, MGA, OPI, PGD, RRN, RME, ROX, RSC, RTF, RTT, SGF, SKN, SNF, SPT, SPT, SRB, STP, SWI, SWI, TAF, THO, UME, YAF, YAP, YAP MET, MMS, MMS, MRE, RAD, RAD, RAD, RAD, RAD, RAD, XRS AKR, CHO, CHO, DAP, ERG, ERG, ERG, ERG, MGA, OPI ASC, BCK, CCS, CTR, CYS, NBP, REG, SKN, SNF, SOD, SOD, YAP, YAP AVT, CWH, KCS, TFP, TFP, VMA, VMA, VMA DIA, MAP, PRE, RAD, VPS, VPS, VPS CYS, ILV, PRO, TRP, TRP, TRP AVT, CTR, MUP, TAT ANP, OCH GND, RPE ARD, BEM, BIM, BUB, BUD, BUD, BUR, CDC, CIK, CSM, CTF, ENV, FYV, GEP, GET, HTZ, KRE, MTC, NAT, NAT, NCE, NPT, NRP, NUP, ORM, PHO, RAI, RCY, REF, RGI, RNR, RPLB, RPLA, RPLB, RTC, SBH, YIM YDRW, YDRC, YHRW, YNRC, YORWUnknown functionsBatova et al. BMC Genomics , : http:biomedcentral-Page ofTable GO-terms substantially enriched in the genes expected for CTBT tolerance (SGD GO-termFinder).GO_term response to stimulus transcription response to anxiety DNA repair organelle JW74 site organization (mitochondrion) FrequencyP-value .E- .E- .E-.Table GO-terms substantially enriched in the fold induced or repressed genes (SGD GO-termFinder).GO_term preribosome nucleus mitochondrion RNA polymerase 
complex response to oxidative pressure monosaccharide catabolic approach response to chemical stimulus FrequencyP-value .E-E- .E- .E- .E- .ERG, ERG), fatty acid (MGA) and phospholipid biosynthesis (CHO, CHO, OPI). In addition to superoxide dismutase encoding genes, SOD and SOD, which have a primary role in superoxide radical detoxification, other genes inved in oxidative stress response were also identified. The CCS gene encodes the precise copper chaperone delivering the copper to Sod. The CTR gene is coding for a high affinity copper transporter in the pla.E). The largest group of strains hypersensitive to CTBT contained deletions in genes for mitochondrial biogenesis and functions, which includes DNA replication (MIP), mRNA processing (MRS), protein synthesis and processing (AFG, DIA, MRPL, MRPS, MSY, MTG, OCT, PCP, RML, RSM, TUF), respiration (CYT), ATP synthesis (ATP, ATP, ATP, ATP), FeS protein biosynthesis (ISA, ISA), superoxide dismutation (SOD, SOD) and other individuals. In the second biggest group were mutants in genes inved in gene expression thus hinting at an acute transcriptional response to CTBT. Identified genes are inved in chromatin remodeling (ARP, HOS, HTZ, RSC, SGF, SNF, SWI, SWI, YAF), transcription (CTK, MED, ROX, RRN, RTF, SPT, SPT, SRB, TAF, THO) or encode transcription factors inved in oxidative tension response (YAP, YAP, SKN) and lipid biosynthesis (OPI). We identified at the least CTBT sensitive strains containing deletion in DNA repair genes, which includes those inved in homologous recombination and repair (MMS, MMS, RAD, RAD, RAD, RAD), post replication repair (RAD, RAD), double strand break repair (MRE, XRS), excision repair (MET) and others. Importantly, deletion of genes encoding functions in lipid metabolism also impaired CTBT tolerance. These are inved in ergosterol (DAP, ERG, ERG,Table Functions of chosen genes deleted in CTBT sensitive mutant strainsFunction Mitochondrial functions Chromatin remodeling and transcription DNA repair Lipid metabolism Tension response and PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27382054?dopt=Abstract signal transduction Vacuolar functions Protein sorting and degradation Amino acid metabolism Transport Mannosyl transferases Pentose phosphate pathway Ungrouped Gene (ORF) name ADH, AFG, ATP, ATP, ATP, ATP, CIT, CYT, DIA, IMP, ISA, ISA, MAS, MDM, MDM, MGM, MIP, MRPL, MRPS, MRS, MSY, MTG, OCT, PCP, PDC, PHB, RML, RSM, SOD, SOD, TUF, YDRW, YGLW ARP, CDC, CTK, HOS, HPR, IES, MED, MGA, OPI, PGD, RRN, RME, ROX, RSC, RTF, RTT, SGF, SKN, SNF, SPT, SPT, SRB, STP, SWI, SWI, TAF, THO, UME, YAF, YAP, YAP MET, MMS, MMS, MRE, RAD, RAD, RAD, RAD, RAD, RAD, XRS AKR, CHO, CHO, DAP, ERG, ERG, ERG, ERG, MGA, OPI ASC, BCK, CCS, CTR, CYS, NBP, REG, SKN, SNF, SOD, SOD, YAP, YAP AVT, CWH, KCS, TFP, TFP, VMA, VMA, VMA DIA, MAP, PRE, RAD, VPS, VPS, VPS CYS, ILV, PRO, TRP, TRP, TRP AVT, CTR, MUP, TAT ANP, OCH GND, RPE ARD, BEM, BIM, BUB, BUD, BUD, BUR, CDC, CIK, CSM, CTF, ENV, FYV, GEP, GET, HTZ, KRE, MTC, NAT, NAT, NCE, NPT, NRP, NUP, ORM, PHO, RAI, RCY, REF, RGI, RNR, RPLB, RPLA, RPLB, RTC, SBH, YIM YDRW, YDRC, YHRW, YNRC, YORWUnknown functionsBatova et al. BMC Genomics , : http:biomedcentral-Page ofTable GO-terms drastically enriched in the genes expected for CTBT tolerance (SGD GO-termFinder).GO_term response to stimulus transcription response to strain DNA repair organelle organization (mitochondrion) FrequencyP-value .E- .E- .E-.Table GO-terms considerably enriched within the fold induced or repressed genes (SGD GO-termFinder).GO_term preribosome nucleus mitochondrion RNA polymerase complex response to oxidative strain monosaccharide catabolic approach response to chemical stimulus FrequencyP-value .E-E- .E- .E- .E- .ERG, ERG), fatty acid (MGA) and phospholipid biosynthesis (CHO, CHO, OPI). In conjunction with superoxide dismutase encoding genes, SOD and SOD, which possess a key function in superoxide radical detoxification, other genes inved in oxidative anxiety response had been also identified. The CCS gene encodes the specific copper chaperone delivering the copper to Sod. The CTR gene is coding for any high affinity copper transporter with the pla.