The truncated CCD2 protein had been cleaved in between Q125 and M126. Presented the noticed cleavage right away before M126, we created a new assemble (CCD3) spanning residues M126-A207
CCD1 and CCD2 ended up equally purified by GST pull down, on column TEV cleavage and anion trade (Determine 2). SDS-Web page investigation of equally recombinant proteins indicated the presence…