Right after addition of the substrate answer for 20 min, the response was terminated by including quit remedy and the shade intensity was measured using a spectrophotometer at 450 nm (Tecan Team Ltd. Switzerland)
C2C12 cells grown in six-well plates to 30% confluence were transfected with 1 ng of vector, TTR and MYOG shRNA construct per effectively utilizing transfection reagent and transfection medium (Santa…