The 59UTR sequence was inserted amongst the SV40 promoter and the luciferase open up reading frame
The mouse fibroblast stable cells NIH3T3Neo (mock-transfected), the NIH3T3Ras, NIH3T3Src, and NIH3T3ErbB2 (stably expressing the constitutively active oncogene H-Ras-V12, Src and ErbB2 respectively) have been attained from Dr. Stephane Richard…