Figure 3-B shows the final results of the western blot detecting the expression of all the mutants in the osteoblast cells. In the C52A/C53A and Cys-much less mutants (see lanes 2 and 4), the lower molecular-mass type was detected

The experimental particulars are described in past reviews . Briefly, overall proteins were extracted from the osteoblast cells which co-expressed IFITM5 and FKBP11FLAG utilizing a full protein extraction kit (BioChain…