Name : Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein
Product Source :
Recombinant Biotinylated Human Fc gamma RIIIB/CD16b (NA1) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus. It contains Gly17-Ser200.[Accession | AAA35881.1]
Molecular Weight :
The protein has a predicted MW of 23.81 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Fc gamma RIIIB (NA1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human Fc gamma RIIIB (NA1) is greater than 95% as determined by SEC-HPLC. SPR Data Biotinylated Human Fc gamma RIIIB (NA1), His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.00 μM as determined in SPR assay (Biacore T200).
Background :
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Synonyms :
CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10
References & Citations :
(1)Qiu W Q , Bruin D D , Brownstein B H , et al. Organization of the human and mouse low-affinity Fc gamma R genes: duplication and recombination[J]. Science, 248.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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