Name : Biotinylated Human FGFR2 alpha (IIIb) Protein
Product Source :
Recombinant Biotinylated Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg22-Glu378.[Accession | P21802-3]
Molecular Weight :
The protein has a predicted MW of 42.5 kDa. Due to glycosylation, the protein migrates to 70-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA.
Background :
Four distinct genes encoding closely related FGF receptors, FGF R1 – 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 – 3 are generated by alternative splicing of the mRNAs.
Synonyms :
FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662
References & Citations :
(1)Katoh, Masaru. FGFR inhibitors: Effects on cancer cells, tumor microenvironment and whole-body homeostasis (Review)[J]. International Journal of Molecular Medicine, 2016.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD73/5′-Nucleotidase Protein
LOX-1/OLR1 Protein
Popular categories:
Ubiquitin-Specific Protease 3
CCL25