Name : Human Coagulation factor XI Protein
Product Source :
Recombinant Human Coagulation factor XI Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu19-Val625.[Accession | P03951-1]
Molecular Weight :
The protein has a predicted MW of 69.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Coagulation factor XI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Coagulation factor XI is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Coagulation factor XI, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA.
Background :
Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.
Synonyms :
Coagulation factor XI; FXI; PTA; F11;
References & Citations :
(1) Puy C, Rigg RA, McCarty OJ. The hemostatic role of factor XI. Thromb Res. 2016 May;141 Suppl 2(Suppl 2):S8-S11. doi: 10.1016/S0049-3848(16)30354-1. PMID: 27207433; PMCID: PMC6135087.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CES2/carboxylesterase 2 Protein
IFN-gamma R2 Protein
Popular categories:
Frizzled
Toll Like Receptor 5