Name : Biotinylated Human ANGPT2/Angiopoietin-2 Protein
Product Source :
Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Lys275-Phe496.[Accession | O15123-1]
Molecular Weight :
The protein has a predicted MW of 28.4 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human ANGPT2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Biotinylated Human ANGPT2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-ANGPT2 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). SPR Data Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 μM as determined in SPR assay (Biacore T200).
Background :
Angiopoietin-2 (ANG-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature ANG-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
Synonyms :
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
References & Citations :
(1)Wu X , Giobbie-Hurder A , Liao X , et al. Angiopoietin-2 as a Biomarker and Target for Immune Checkpoint Therapy[J]. Cancer Immunology Research, 2017, 5(1):17-28.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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