Name : Biotinylated Rat GDF15 Protein (Primary Amine Labeling)
Product Source :
Recombinant Biotinylated Rat GDF15 Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the N-terminus. It contains Ser189-Ala303.[Accession | Q9Z0J6]
Molecular Weight :
The protein has a predicted MW of 13.68 kDa. The protein migrates to 14-17 kDa under reduced (R) condition and 25-30 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Supplied as 0.22 μm filtered solution in 50mM HAc.
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Rat GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. ELISA Data Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA.
Background :
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Synonyms :
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
References & Citations :
(1)Baek SJ, Eling T. Growth Differentiation Factor 15 (GDF15): A survival protein with therapeutic potential in metabolic diseases. Pharmacol Ther. 2019 Jun;198:46-58. doi: 10.1016/j.pharmthera.2019.02.008. Epub 2019 Feb 18. PMID: 30790643; PMCID: PMC7196666.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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