Name : Cynomolgus BDCA-2 Protein
Product Source :
Recombinant Cynomolgus BDCA-2 Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Tyr48-Ile212.[Accession | A0A2K5UWP4]
Molecular Weight :
The protein has a predicted MW of 44.99 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus BDCA-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus BDCA-2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus BDCA-2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA.
Background :
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Synonyms :
BDCA-2; BDCA2; CLECSF11; CLECSF7; DLEC; HECL; CLEC4C; CD303; CD303; DLEC;
References & Citations :
(1) Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol. 2000 Dec 1;165(11):6037-46. doi: 10.4049/jimmunol.165.11.6037. PMID: 11086035.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
HER3 Protein
CD40L/CD154/TRAP Protein
Popular categories:
Ubiquitin-Specific Protease
Glycoprotein Hormone alpha-2 (GPHA2)