Name : Human Nectin-2/CD112 Protein
Product Source :
Recombinant Human Nectin-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln32-Leu360.[Accession | Q92692-2]
Molecular Weight :
The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Nectin-2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test). SPR Data Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). SPR Data Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 μM as determined in SPR assay (Biacore T200).
Background :
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Synonyms :
CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
References & Citations :
(1) Bekes I, Löb S, Holzheu I, Janni W, Baumann L, Wöckel A, Wulff C. Nectin-2 in ovarian cancer: How is it expressed and what might be its functional role? Cancer Sci. 2019 Jun;110(6):1872-1882. doi: 10.1111/cas.13992. Epub 2019 May 2. PMID: 30843637; PMCID: PMC6549928.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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