Name : Human Nectin-3/CD113 Protein
Product Source :
Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Gly58-Asp400.[Accession | Q9NQS3]
Molecular Weight :
The protein has a predicted MW of 63.63 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Nectin-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Nectin-3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24μg/ml determined by ELISA.
Background :
The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
Synonyms :
CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
References & Citations :
(1) Inagaki, M. Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development[J]. Development, 132(7):1525-1537.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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