Name : Human NKG2A&CD94 Protein
Product Source :
Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Arg100-Ala219(NKG2A)&Ser34-Ile179(CD94).[Accession | P26715-1(NKG2A)&Q13241-1(CD94)]
Molecular Weight :
The protein has a predicted MW of 17.9 kDa (CD94)&41.1 kDa (NKG2A). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human NKG2A&CD94, hFc tag at 1μg/ml (100μl/Well) on Fc Antibody (5μg/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Ab., hFc Tag with the EC50 of 22.4ng/ml determined by ELISA.
Background :
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Synonyms :
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
References & Citations :
(1) Hò GT, Celik AA, Huyton T, Hiemisch W, Blasczyk R, Simper GS, Bade-Doeding C. NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain. Int J Mol Sci. 2020 Jun 19;21(12):4362. doi: 10.3390/ijms21124362. PMID: 32575403; PMCID: PMC7352787.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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