Name : Cynomolgus NKp46/NCR1/CD335 Protein
Product Source :
Recombinant Cynomolgus NKp46/NCR1/CD335 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Pro22-Asn254.[Accession | Q95JB9]
Molecular Weight :
The protein has a predicted MW of 27.4 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus NKp46 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus NKp46 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus NKp46, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NKp46 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Background :
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
Synonyms :
CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094
References & Citations :
(1)Glasner A, Isaacson B, Viukov S, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression.[J]. Sci Rep, 2017, 7(1):13090.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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