Name : Human Notch 3 Protein
Product Source :
Recombinant Human Notch 3 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala40-Glu467.[Accession | Q9UM47]
Molecular Weight :
The protein has a predicted MW of 71.53 kDa. Due to glycosylation, the protein migrates to 75-83 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Background :
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs
Synonyms :
CADASIL; Notch homolog 3; Notch3; Notch-3
References & Citations :
(1)Wu Y , Cain-Hom C , Choy L , et al. Therapeutic antibody targeting of individual Notch receptors[J]. NATURE, 2010, 464(7291):1052-1057.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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