Name : Human NRG1 Beta 1 Protein
Product Source :
Recombinant Human NRG1 Beta 1 Protein is expressed from HEK293 with hFc tag at the N-terminus. It contains Ser2-Lys246.[Accession | Q02297-6]
Molecular Weight :
The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human NRG1 Beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Her4, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 18.7ng/ml determined by ELISA.
Background :
Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.
Synonyms :
Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A
References & Citations :
Liew H, Kim YM, Choi HS, Jang AR, Churchill D, Lee SH, Suh YH. Soluble Neuregulin-1 from Microglia Enhances Amyloid Beta-induced Neuronal Death. CNS Neurol Disord Drug Targets. 2016;15(8):918-926. doi: 10.2174/1871527315666160815160505. PMID: 27577739.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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