Name : Human Neuropilin-1 Protein
Product Source :
Recombinant Human Neuropilin-1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe22-Lys644.[Accession | NP_001019799.1]
Molecular Weight :
The protein has a predicted MW of 70.97 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Neuropilin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Neuropilin-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human VEGF165, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57μg/ml determined by ELISA.
Background :
Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta.
Synonyms :
BDCA4; NP1; Neuropilin-1; NRP1; CD304; VEGF165R; Neuropilin 1; NRP; Transmembrane Receptor; CD304 Antigen
References & Citations :
Chaudhary B, Khaled YS, Ammori BJ, Elkord E. Neuropilin 1: function and therapeutic potential in cancer. Cancer Immunol Immunother. 2014 Feb;63(2):81-99. doi: 10.1007/s00262-013-1500-0. Epub 2013 Nov 22. PMID: 24263240.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CHI3L1 Protein
B7-2/CD86 Protein
Popular categories:
VEGF-A
CD200R