Name : Human Oncostatin M/OSM Protein
Product Source :
Recombinant Human Oncostatin M/OSM Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala26-Arg221.[Accession | P13725]
Molecular Weight :
The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human OSM on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Human OSM is greater than 95% as determined by SEC-HPLC. SPR Data Human LIF R, His Tag immobilized on CM5 Chip can bind Human OSM, His Tag with an affinity constant of 0.662 μM as determined in SPR assay (Biacore T200).
Background :
OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Synonyms :
Oncostatin M; OSM; MGC20461
References & Citations :
(1)Junk D J , Bryson B L , Smigiel J M , et al. Oncostatin M promotes cancer cell plasticity through cooperative STAT3-SMAD3 signaling[J]. Oncogene, 2017.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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