Name : Human OSMR-GPL fusion Protein
Product Source :
Recombinant Human OSMR-GPL fusion Protein is expressed from HEK293 with His tag at the C-terminus. It contains Met1-Ala428 (OSMR) and Leu21-Glu225 (GPL).[Accession | Q99650-1(OSMR)&Q8NI17-1(GPL)]
Molecular Weight :
The protein has a predicted MW of 75.84 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human OSMR-GPL fusion Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human OSMR-GPL fusion Protein, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
Background :
OSMR involvement, together with Gp130-like receptor (GPL), also known as IL-31R, in the formation of a functional IL-31 receptor complex. OSMR-GPL fusion protein was designed to contain the domains of OSMR and GPL required for high affinity IL-31 binding.
Synonyms :
OSMR-GPL
References & Citations :
[1] M, Grant FJ, Lockwood L, Levin SD, LeCiel C, Waggie K, Day H, Topouzis S, Kramer J, Kuestner R, Chen Z, Foster D, Parrish-Novak J, Gross JA. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat Immunol. 2004 Jul;5(7):752-60.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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