Name : Human PAH (D415N) Protein
Product Source :
Recombinant Human PAH (D415N) Protein is expressed from Baculovirus-Insect Cells with His tag at the N-terminus. It contains Met1-Lys452 (D415N).[Accession | P00439]
Molecular Weight :
The protein has a predicted MW of 52.82 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 0.5M NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PAH (D415N) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PAH (D415N) is greater than 95% as determined by SEC-HPLC.
Background :
Phenylalanine hydroxylase (PAH) is a member of aromatic amino acid hydroxylase (AAAHs) family, and catalyze phenylalanine (Phe) into tyrosine (Tyr). PAH is also an allosteric enzyme that maintains phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria, an inborn error of amino acid metabolism.
Synonyms :
PH; PKU; PKU1;
References & Citations :
(1) Ren G, Dong Z, Liu C, Liu Y, Luan Z, Liu Q, Bao X, Wang S. [The expression of phenylalanine hydroxylase in the brain of ragworm Neanthes japonica (Polychaeta, Annelida)]. Sheng Wu Gong Cheng Xue Bao. 2016 Apr 25;32(4):518-526. Chinese. doi: 10.13345/j.cjb.150550. PMID: 28853273.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PAM Protein
GRO-alpha/CXCL1 Protein
Popular categories:
PSBG1 Protein/CD66f
ADAM23