Name : Human PHOSPHO1 Protein
Product Source :
Recombinant Human PHOSPHO1 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Met1-Cys267.[Accession | Q8TCT1-1]
Molecular Weight :
The protein has a predicted MW of 30.67 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 300mM L-Arginine (pH 7.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human PHOSPHO1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human PHOSPHO1 is greater than 95% as determined by SEC-HPLC.
Background :
PHOSPHO1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralisation.
Synonyms :
Phosphocholine phosphatase-1
References & Citations :
Roberts SJ, Stewart AJ, Schmid R, Blindauer CA, Bond SR, Sadler PJ, Farquharson C. Probing the substrate specificities of human PHOSPHO1 and PHOSPHO2. Biochim Biophys Acta. 2005 Aug 31;1752(1):73-82. doi: 10.1016/j.bbapap.2005.06.009. PMID: 16054448.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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