Name : Human CHRDL1 Protein
Product Source :
Recombinant Human CHRDL1 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Glu28-Cys456.[Accession | Q9BU40-1]
Molecular Weight :
The protein has a predicted MW of 50 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 90% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CHRDL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%. ELISA Data Immobilized Human CHRDL1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Background :
CHRDL1 (Chordin-like 1) is a secreted protein that acts as an antagonist of bone morphogenetic protein (BMP). BMP plays a role as an activator of BMP receptor II (BMPR II), which mediates extracellular to intracellular signal transmission and is involved in carcinogenesis and metastasis.The hypermethylation of the CHRDL1 promoter in gastric cancer, which induced low expression of CHRDL1 and decreased its secretion to the supernatant.
Synonyms :
Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1; CHL; Chordin-like 1; CRDL1; dA141H5.1; VOPT
References & Citations :
(1) Pei YF, Zhang YJ, Lei Y, Wu WD, Ma TH, Liu XQ. Hypermethylation of the CHRDL1 promoter induces proliferation and metastasis by activating Akt and Erk in gastric cancer. Oncotarget. 2017 Apr 4;8(14):23155-23166. doi: 10.18632/oncotarget.15513. Erratum in: Oncotarget. 2017 Jul 18;8(29):48526. Wu, Ding-Wei [corrected to Wu, Wei-ding]. PMID: 28423564; PMCID: PMC5410293.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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