Name : Cynomolgus CD44 Protein
Product Source :
Recombinant Cynomolgus CD44 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln21-Pro220 & Gln387-Ala428.[Accession | A0A2K5VJE0]
Molecular Weight :
The protein has a predicted MW of 27.77 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus CD44 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus CD44 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus CD44, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD44 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Background :
CD44 is a hyaluronan binding cell surface signal transducing receptor that influences motility, cell survival and proliferation as well as the formation of tumor microenvironment. CD44 contains two variable regions encoded by variable exons. Alternative splicing, which is often deregulated in cancer, can produce various isoforms of CD44 with properties that may have different tissue specific effects and therefore even diverse effects on cancer progression
Synonyms :
CD44; CDw44; Epican; ECMR-III; LHR; MDU2; MDU3; MIC4; PGP-I; CSPG8; HCELL; HUTCH-I; IN; MC56
References & Citations :
(1) Prochazka L, Tesarik R, Turanek J. Regulation of alternative splicing of CD44 in cancer. Cell Signal. 2014 Oct;26(10):2234-9. doi: 10.1016/j.cellsig.2014.07.011. Epub 2014 Jul 13. PMID: 25025570.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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