Name : Mouse CHODL Protein

Product Source :
Recombinant Mouse CHODL Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Arg22-Asn216.[Accession | Q9CXM0-1]

Molecular Weight :
The protein has a predicted MW of 48.7 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse CHODL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CHODL is greater than 95% as determined by SEC-HPLC.

Background :
chondrolectin (CHODL) is commonly overexpressed in the majority of lung cancers, indicating a possible correlation between CHODL and metastasis of lung cancer cells. the expression of CHODL is significantly decreased in HCC clinical samples and in HCC cell lines. Overexpression of CHODL in SMMC7721 cells with a lentiviral vector increased SMMC7721 cell migration and invasion.

Synonyms :
Chondrolectin; CHODL; C21orf68; 3110074E07Rik; MT75; PRED12; FLJ12627

References & Citations :
(1) Huang Z, Zhang N, Li W, Cao J, Zhang L, Chen Y. Expression of CHODL in hepatocellular carcinoma affects invasion and migration of liver cancer cells. Oncol Lett. 2017 Feb;13(2):715-721. doi: 10.3892/ol.2016.5466. Epub 2016 Dec 6. PMID: 28356950; PMCID: PMC5351393.

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