Name : Canine CEACAM-1/CD66a Protein

Product Source :
Recombinant Canine CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln35-Gly424.[Accession | XP_038509497.1]

Molecular Weight :
The protein has a predicted MW of 43.62 kDa. Due to glycosylation, the protein migrates to 68-88 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Canine CEACAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Canine CEACAM-1 is greater than 95% as determined by SEC-HPLC.

Background :
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro.

Synonyms :
BGP; BGP1; BGPI; CEACAM1; CD66a

References & Citations :
(1)Piancone F, Saresella M, Marventano I, La Rosa F, Caputo D, Mendozzi L, Rovaris M, Clerici M. A Deficit of CEACAM-1-Expressing T Lymphocytes Supports Inflammation in Primary Progressive Multiple Sclerosis. J Immunol. 2019 Jul 1;203(1):76-83. doi: 10.4049/jimmunol.1801625. Epub 2019 May 29. PMID: 31142599.

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