Name : Human CEACAM-1/CD66a Protein
Product Source :
Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln35-Gly428.[Accession | P13688-1]
Molecular Weight :
The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CEACAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CEACAM-1 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). SPR Data Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 μM as determined in SPR assay (Biacore T200).
Background :
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro.
Synonyms :
BGP; BGP1; BGPI; CEACAM1; CD66a
References & Citations :
(1)Piancone F, Saresella M, Marventano I, La Rosa F, Caputo D, Mendozzi L, Rovaris M, Clerici M. A Deficit of CEACAM-1-Expressing T Lymphocytes Supports Inflammation in Primary Progressive Multiple Sclerosis. J Immunol. 2019 Jul 1;203(1):76-83. doi: 10.4049/jimmunol.1801625. Epub 2019 May 29. PMID: 31142599.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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