Name : Human CEACAM-3 Protein
Product Source :
Recombinant Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys35-Gly155.[Accession | P40198-1]
Molecular Weight :
The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CEACAM-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CEACAM-7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.
Background :
The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.
Synonyms :
CEACAM3; CD66d; CGM1; CEA;
References & Citations :
Schmitter T, Pils S, Sakk V, Frank R, Fischer KD, Hauck CR. The granulocyte receptor carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) directly associates with Vav to promote phagocytosis of human pathogens. J Immunol. 2007 Mar 15;178(6):3797-805. doi: 10.4049/jimmunol.178.6.3797. PMID: 17339478.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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