Name : Human CEACAM-7 Protein
Product Source :
Recombinant Human CEACAM-7 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Thr36-Ser242.[Accession | Q14002-1]
Molecular Weight :
The protein has a predicted MW of 50.11 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CEACAM-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CEACAM-7 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CEACAM-3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA.
Background :
Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to a group of mammalian immunoglobulin-related glycoproteins. They are involved in cell–cell recognition and modulate cellular processes that range from the shaping of tissue architecture and neovascularization to the regulation of insulin homeostasis and T-cell proliferation. CEACAM7 (CGM2), a member of the CEA family of proteins with expression restricted to the colon and pancreas, as a potential CAR T-cell target for PDAC.
Synonyms :
CGM2; CEACAM7; CEA
References & Citations :
(1) Kuespert K, Pils S, Hauck CR. CEACAMs: their role in physiology and pathophysiology. Curr Opin Cell Biol. 2006 Oct;18(5):565-71. doi: 10.1016/j.ceb.2006.08.008. Epub 2006 Aug 17. PMID: 16919437; PMCID: PMC7127089.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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