Name : Human Complement Factor D/CFD Protein
Product Source :
Recombinant Human Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile26-Ala253.[Accession | P00746-1]
Molecular Weight :
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 26-27 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Complement Factor D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Complement Factor D is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Complement Factor D, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Background :
Complement Factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement Factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.
Synonyms :
Adipsin; C3 convertase activator; Complement Factor D; CFD; PFD; DF; ADN; Factor D; AMBP-1; EC 3.4.21; EC 3.4.21.46
References & Citations :
(1) Abrera-Abeleda MA, Xu Y, Pickering MC, Smith RJ, Sethi S. Mesangial immune complex glomerulonephritis due to complement Factor D deficiency. Kidney Int. 2007 Jun;71(11):1142-7. doi: 10.1038/sj.ki.5002235. Epub 2007 Apr 4. PMID: 17410102.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
CD3D Proteinsite
CISD1 Proteinmanufacturer
Popular categories:
SMAD4
IL-2R alpha